MathWorks Inc math function blocks
Math Function Blocks, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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α globin (Santa Cruz Biotechnology)

Santa Cruz Biotechnology α globin
$p66α directly interacts with CP2c and CP2b to repress globin gene expression. ( A ) ONPG analysis shows interaction of p66α with CP2c and CP2b. n = 2. neg; negative control (p66α alone), pos; positive control (p53/T antigen). ( B ) Co-IP analysis shows the exogenous p66α interaction with endogenous CP2c and CP2b, but not with Pias1. ( C ) Luc reporter analysis shows p66α-mediated repression of the CBP-driven transcriptional activity. n = 4. CP2c tet; a synthetic promoter containing only tetrameric CP2c half-binding sites linked to the β-globin TATA box, ΔGata1; the <t>α-globin</t> gene promoter with the deleted Gata1 binding site, mα-globin; the intact α-globin gene promoter, and mut CP2c; the Gata1 proximal enhancer with mutations in the CP2c binding sites. ( D ) Immunoblot analysis of the expression of NuRD proteins in differentiating MEL cells in vitro . The values normalized to β-tubulin or β-actin are shown as mean ± SEM; n = 2. ( E ) Scatterplot of the gated mouse bone marrow cells (left), and immunoblot analysis (right) of the Mbd2, Mbd3, and p66α expression in each cell fraction.$
α Globin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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simulink math block (MathWorks Inc)

MathWorks Inc simulink math block
$p66α directly interacts with CP2c and CP2b to repress globin gene expression. ( A ) ONPG analysis shows interaction of p66α with CP2c and CP2b. n = 2. neg; negative control (p66α alone), pos; positive control (p53/T antigen). ( B ) Co-IP analysis shows the exogenous p66α interaction with endogenous CP2c and CP2b, but not with Pias1. ( C ) Luc reporter analysis shows p66α-mediated repression of the CBP-driven transcriptional activity. n = 4. CP2c tet; a synthetic promoter containing only tetrameric CP2c half-binding sites linked to the β-globin TATA box, ΔGata1; the <t>α-globin</t> gene promoter with the deleted Gata1 binding site, mα-globin; the intact α-globin gene promoter, and mut CP2c; the Gata1 proximal enhancer with mutations in the CP2c binding sites. ( D ) Immunoblot analysis of the expression of NuRD proteins in differentiating MEL cells in vitro . The values normalized to β-tubulin or β-actin are shown as mean ± SEM; n = 2. ( E ) Scatterplot of the gated mouse bone marrow cells (left), and immunoblot analysis (right) of the Mbd2, Mbd3, and p66α expression in each cell fraction.$
Simulink Math Block, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s-function block (MathWorks Inc)

MathWorks Inc s-function block
$p66α directly interacts with CP2c and CP2b to repress globin gene expression. ( A ) ONPG analysis shows interaction of p66α with CP2c and CP2b. n = 2. neg; negative control (p66α alone), pos; positive control (p53/T antigen). ( B ) Co-IP analysis shows the exogenous p66α interaction with endogenous CP2c and CP2b, but not with Pias1. ( C ) Luc reporter analysis shows p66α-mediated repression of the CBP-driven transcriptional activity. n = 4. CP2c tet; a synthetic promoter containing only tetrameric CP2c half-binding sites linked to the β-globin TATA box, ΔGata1; the <t>α-globin</t> gene promoter with the deleted Gata1 binding site, mα-globin; the intact α-globin gene promoter, and mut CP2c; the Gata1 proximal enhancer with mutations in the CP2c binding sites. ( D ) Immunoblot analysis of the expression of NuRD proteins in differentiating MEL cells in vitro . The values normalized to β-tubulin or β-actin are shown as mean ± SEM; n = 2. ( E ) Scatterplot of the gated mouse bone marrow cells (left), and immunoblot analysis (right) of the Mbd2, Mbd3, and p66α expression in each cell fraction.$
S Function Block, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/s-function block/product/MathWorks Inc
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Image Search Results

$p66α directly interacts with CP2c and CP2b to repress globin gene expression. ( A ) ONPG analysis shows interaction of p66α with CP2c and CP2b. n = 2. neg; negative control (p66α alone), pos; positive control (p53/T antigen). ( B ) Co-IP analysis shows the exogenous p66α interaction with endogenous CP2c and CP2b, but not with Pias1. ( C ) Luc reporter analysis shows p66α-mediated repression of the CBP-driven transcriptional activity. n = 4. CP2c tet; a synthetic promoter containing only tetrameric CP2c half-binding sites linked to the β-globin TATA box, ΔGata1; the α-globin gene promoter with the deleted Gata1 binding site, mα-globin; the intact α-globin gene promoter, and mut CP2c; the Gata1 proximal enhancer with mutations in the CP2c binding sites. ( D ) Immunoblot analysis of the expression of NuRD proteins in differentiating MEL cells in vitro . The values normalized to β-tubulin or β-actin are shown as mean ± SEM; n = 2. ( E ) Scatterplot of the gated mouse bone marrow cells (left), and immunoblot analysis (right) of the Mbd2, Mbd3, and p66α expression in each cell fraction.$

Journal: Nucleic Acids Research

Article Title: Mbd2-CP2c loop drives adult-type globin gene expression and definitive erythropoiesis

doi: 10.1093/nar/gky193

Figure Lengend Snippet: p66α directly interacts with CP2c and CP2b to repress globin gene expression. ( A ) ONPG analysis shows interaction of p66α with CP2c and CP2b. n = 2. neg; negative control (p66α alone), pos; positive control (p53/T antigen). ( B ) Co-IP analysis shows the exogenous p66α interaction with endogenous CP2c and CP2b, but not with Pias1. ( C ) Luc reporter analysis shows p66α-mediated repression of the CBP-driven transcriptional activity. n = 4. CP2c tet; a synthetic promoter containing only tetrameric CP2c half-binding sites linked to the β-globin TATA box, ΔGata1; the α-globin gene promoter with the deleted Gata1 binding site, mα-globin; the intact α-globin gene promoter, and mut CP2c; the Gata1 proximal enhancer with mutations in the CP2c binding sites. ( D ) Immunoblot analysis of the expression of NuRD proteins in differentiating MEL cells in vitro . The values normalized to β-tubulin or β-actin are shown as mean ± SEM; n = 2. ( E ) Scatterplot of the gated mouse bone marrow cells (left), and immunoblot analysis (right) of the Mbd2, Mbd3, and p66α expression in each cell fraction.

Article Snippet: After blocking with PBS containing 5% nonfat dry milk in a solution of 0.1% Tween 20, membranes were incubated with the following antibodies: CP2c (Cosmogentec), CP2b (Peptron), Pias1 (Lifespan LS-C90260), EGFP (Abcam ab5449), HA (Abcam ab49969), Flag (Sigma Aldrich F1804), His (Santa Cruz sc-804), GST (Bodytech), Myc (Abcam ab9106), Chd4 (Abcam ab70469), Mbd2 (Abcam ab38646), Mbd3 (Abcam ab157464), p66α (Abcam ab87663), RbAp48 (Abcam ab47456), Hdac1 (Santa Cruz sc-6298), Mta1 (Santa Cruz sc-13142), α-globin (Santa Cruz sc-31110), and β-globin (Santa Cruz sc-31116).

Techniques: Gene Expression, Negative Control, Positive Control, Co-Immunoprecipitation Assay, Activity Assay, Binding Assay, Western Blot, Expressing, In Vitro

Mbd2 downregulation is crucial for globin expression and terminal erythroid differentiation. ( A ) Immunoblot analysis of the Mbd2, Mbd3, and p66α expression in Mbd2 KD, Mbd3 KD and Mbd DKD cells during HMBA-induced differentiation of MEL cells. ( B ) Immunoblot analysis of the α- and β-globin expression in uninduced (d0) and in HMBA-induced (d3) Mbd2 KD and Mbd3 KD MEL cells (adapted from the time-course data in ). The values normalized to β-tubulin are shown as mean ± SEM; n = 2. ( C ) RT-qPCR analysis of the α-globin expression in Mbd2 KD cells. Values normalized to Gapdh are shown as mean ± SEM; n = 3. ( D ) RT-qPCR analysis of the expression of erythroid markers in uninduced (d0) and in HMBA-induced (d3) Mbd2 KD and Mbd3 KD MEL cells. n = 2. ( E ) Functional hemoglobin synthesis analysis by benzidine staining. Fraction of benzidine stain-positive cells was scored. Values are mean ± SEM; n = 4. Bar = 100 μm.

Journal: Nucleic Acids Research

Article Title: Mbd2-CP2c loop drives adult-type globin gene expression and definitive erythropoiesis

doi: 10.1093/nar/gky193

Figure Lengend Snippet: Mbd2 downregulation is crucial for globin expression and terminal erythroid differentiation. ( A ) Immunoblot analysis of the Mbd2, Mbd3, and p66α expression in Mbd2 KD, Mbd3 KD and Mbd DKD cells during HMBA-induced differentiation of MEL cells. ( B ) Immunoblot analysis of the α- and β-globin expression in uninduced (d0) and in HMBA-induced (d3) Mbd2 KD and Mbd3 KD MEL cells (adapted from the time-course data in ). The values normalized to β-tubulin are shown as mean ± SEM; n = 2. ( C ) RT-qPCR analysis of the α-globin expression in Mbd2 KD cells. Values normalized to Gapdh are shown as mean ± SEM; n = 3. ( D ) RT-qPCR analysis of the expression of erythroid markers in uninduced (d0) and in HMBA-induced (d3) Mbd2 KD and Mbd3 KD MEL cells. n = 2. ( E ) Functional hemoglobin synthesis analysis by benzidine staining. Fraction of benzidine stain-positive cells was scored. Values are mean ± SEM; n = 4. Bar = 100 μm.

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Functional Assay, Staining

ChIP-qPCR profiles of NuRD proteins, CBP proteins, and Gata1/Fog1 to the α-globin promoter during MEL cell differentiation. ( A ) Schematic representation of the α-globin promoter with ChIP-qPCR probes (1–5). GATA; Gata1 binding site, [CNRG-N (5-6) -CNRG]; CP2c binding site, CCAAT; Nfy binding site, TATA; TATA box. ChIP-qPCR profiles of NuRD proteins ( B ), CBP proteins ( D ), and Gata1 and Fog1 ( E ) on the α-globin promoter in uninduced WT (d0 WT), uninduced Mbd2 KD (d0 Mbd2 KD), and HMBA-induced WT (d3 WT) MEL cells. n = 2. IgG and Errc3 are used as internal negative controls. ( C ) Immunoblot analysis of the Ac-Hdac1 and Hdac1 expression during HMBA-induced differentiation of Mbd2 KD and Mbd3 KD MEL cells. Values are normalized to β-tubulin and relative values over WT at d0 are shown as mean ± SEM; n = 2.

Journal: Nucleic Acids Research

Article Title: Mbd2-CP2c loop drives adult-type globin gene expression and definitive erythropoiesis

doi: 10.1093/nar/gky193

Figure Lengend Snippet: ChIP-qPCR profiles of NuRD proteins, CBP proteins, and Gata1/Fog1 to the α-globin promoter during MEL cell differentiation. ( A ) Schematic representation of the α-globin promoter with ChIP-qPCR probes (1–5). GATA; Gata1 binding site, [CNRG-N (5-6) -CNRG]; CP2c binding site, CCAAT; Nfy binding site, TATA; TATA box. ChIP-qPCR profiles of NuRD proteins ( B ), CBP proteins ( D ), and Gata1 and Fog1 ( E ) on the α-globin promoter in uninduced WT (d0 WT), uninduced Mbd2 KD (d0 Mbd2 KD), and HMBA-induced WT (d3 WT) MEL cells. n = 2. IgG and Errc3 are used as internal negative controls. ( C ) Immunoblot analysis of the Ac-Hdac1 and Hdac1 expression during HMBA-induced differentiation of Mbd2 KD and Mbd3 KD MEL cells. Values are normalized to β-tubulin and relative values over WT at d0 are shown as mean ± SEM; n = 2.

Techniques: ChIP-qPCR, Cell Differentiation, Binding Assay, Western Blot, Expressing

Interplay of Gata1/Fog1, Mbd2-NuRD, and CP2c complexes occurs in adjacent Gata1 and CP2c binding sites of erythroid genes. ChIP-qPCR profiles of Gata1 and Fog1 ( A ), Mbd2 and p66α ( B ), and CP2c ( C ) on the α-globin promoter (probes 3 and 4) in the uninduced MEL cells with the siRNA-driven Gata1 or Fog1 KD. n = 2. An insert in (A) shows immunoblots of the expression levels of Gata1 and Fog1. ( D ) Working model of the Gata1/Fog1-NuRD-CP2c axis in the α-globin promoter during HMBA-induced MEL cell differentiation. uMEL; undifferentiated MEL cells, iMEL; HMBA-induced MEL cells.

Journal: Nucleic Acids Research

Article Title: Mbd2-CP2c loop drives adult-type globin gene expression and definitive erythropoiesis

doi: 10.1093/nar/gky193

Figure Lengend Snippet: Interplay of Gata1/Fog1, Mbd2-NuRD, and CP2c complexes occurs in adjacent Gata1 and CP2c binding sites of erythroid genes. ChIP-qPCR profiles of Gata1 and Fog1 ( A ), Mbd2 and p66α ( B ), and CP2c ( C ) on the α-globin promoter (probes 3 and 4) in the uninduced MEL cells with the siRNA-driven Gata1 or Fog1 KD. n = 2. An insert in (A) shows immunoblots of the expression levels of Gata1 and Fog1. ( D ) Working model of the Gata1/Fog1-NuRD-CP2c axis in the α-globin promoter during HMBA-induced MEL cell differentiation. uMEL; undifferentiated MEL cells, iMEL; HMBA-induced MEL cells.

Techniques: Binding Assay, ChIP-qPCR, Western Blot, Expressing, Cell Differentiation

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